Títol | Three-Dimensional Genomic Structure and Cohesin Occupancy Correlate with Transcriptional Activity during Spermatogenesis. |
Tipus de publicació | Journal Article |
Any de publicació | 2019 |
Autors | Vara, C, Paytuví-Gallart, A, Cuartero, Y, Le Dily, F, Garcia, F, Salvà-Castro, J, Gómez-H, L, Julià, E, Moutinho, C, Cigliano, RAiese, Sanseverino, W, Fornas, O, Pendás, AM, Heyn, H, Waters, PD, Marti-Renom, MA, Ruiz-Herrera, A |
Journal | Cell Rep |
Volum | 28 |
Pàgines | 352-367.e9 |
Date Published | 2019 Jul 09 |
ISSN | 2211-1247 |
Resum | Mammalian gametogenesis involves dramatic and tightly regulated chromatin remodeling, whose regulatory pathways remain largely unexplored. Here, we generate a comprehensive high-resolution structural and functional atlas of mouse spermatogenesis by combining in situ chromosome conformation capture sequencing (Hi-C), RNA sequencing (RNA-seq), and chromatin immunoprecipitation sequencing (ChIP-seq) of CCCTC-binding factor (CTCF) and meiotic cohesins, coupled with confocal and super-resolution microscopy. Spermatogonia presents well-defined compartment patterns and topological domains. However, chromosome occupancy and compartmentalization are highly re-arranged during prophase I, with cohesins bound to active promoters in DNA loops out of the chromosomal axes. Compartment patterns re-emerge in round spermatids, where cohesin occupancy correlates with transcriptional activity of key developmental genes. The compact sperm genome contains compartments with actively transcribed genes but no fine-scale topological domains, concomitant with the presence of protamines. Overall, we demonstrate how genome-wide cohesin occupancy and transcriptional activity is associated with three-dimensional (3D) remodeling during spermatogenesis, ultimately reprogramming the genome for the next generation. |
DOI | 10.1016/j.celrep.2019.06.037 |
Alternate Journal | Cell Rep |
ID de PubMed | 31291573 |
PubMed Central ID | PMC6635386 |