Selected publications

Li J. and Boix E. Host Defense RNases as Antiviral agents against enveloped single-stranded RNA Viruses. Virulence (2021) 12:1, 444-469.

Lu, L.;Wei, R.; Bhakta, S.; Waddell, S.J.; Boix, E.Weighted Gene Co-Expression Network Analysis Identifies Key Modules and Hub Genes Associated with Mycobacterial Infection of Human Macrophages. Antibiotics (2021), 10, 97.

Lu, L., Wei, R., Prats-Ejarque, G. Goetz, M, Torrent M &  Boix E. Human RNase3 immune modulation by catalytic-dependent and independent modes in a macrophage-cell line infection model. Cell. Mol. Life Sci. (2020).

Rangel-Muñoz N, Suarez-Arnedo A, Anguita R, Prats-Ejarque G, Osma JF,  Muñoz-Camargo C, Boix E, Cruz JC and Salazar VA Magnetite Nanoparticles Functionalized with RNases against Intracellular Infection of Pseudomonas aeruginosa Pharmaceutics 2020, 12(7), 631;

Li J, Fernández-Millán P, Boix E. Synergism between host defence peptides and
antibiotics against bacterial infections. Curr Top Med Chem. 2020 Mar 3. doi:

Boix E, Acquati F, Leonidas D, Pulido D. Editorial: Role of Ribonucleases in
Immune Response Regulation During Infection and Cancer. Front Immunol. 2020 Feb
19;11:236. doi: 10.3389/fimmu.2020.00236. eCollection 2020.

Prats-Ejarque G, Lu L, Salazar VA, Moussaoui M, Boix E. Evolutionary Trends in
RNA Base Selectivity Within the RNase A Superfamily. Front Pharmacol. 2019 Oct
9;10:1170. doi: 10.3389/fphar.2019.01170.

Salazar VA, Arranz-Trullén J, Prats-Ejarque G, Torrent M, Andreu D, Pulido D,
Boix E. Insight into the Antifungal Mechanism of Action of Human RNase N-terminus
Derived Peptides. Int J Mol Sci. 2019 Sep 14;20(18). pii: E4558. doi:10.3390/ijms20184558.

Lu L, Arranz-Trullén J, Prats-Ejarque G, Pulido D, Bhakta S, Boix E. Human
Antimicrobial RNases Inhibit Intracellular Bacterial Growth and Induce Autophagy
in Mycobacteria-Infected Macrophages. Front Immunol. 2019 Jul 2;10:1500.

Prats-Ejarque G, Li J, Ait-Ichou F, Lorente H, Boix E. Testing a Human
Antimicrobial RNase Chimera Against Bacterial Resistance. Front Microbiol. 2019
Jun 19;10:1357.

Prats-Ejarque G, Blanco JA, Salazar VA, Nogués VM, Moussaoui M, Boix E.
Characterization of an RNase with two catalytic centers. Human RNase6 catalytic
and phosphate-binding site arrangement favors the endonuclease cleavage of
polymeric substrates. Biochim Biophys Acta Gen Subj. 2019 Jan;1863(1):105-117.

Lu L, Li J, Moussaoui M and Boix E Immune modulation by human secreted RNases at the extracellular space.
Front Immunol (2018) 8:1499. 1-17

Pulido D, Prats-Ejarque G, Villalba C, Albacar M, Moussaoui M, Andreu D,
Volkmer R, Torrent M, Boix E. Positional scanning library applied to the human
eosinophil cationic protein/RNase3 N-terminus reveals novel and potent
anti-biofilm peptides. Eur J Med Chem. 2018 May 25;152:590-599.

Muñoz-Camargo C, Salazar VA, Barrero-Guevara L, Camargo S, Mosquera A, Groot
H, Boix E. Unveiling the Multifaceted Mechanisms of Antibacterial Activity of
Buforin II and Frenatin 2.3S Peptides from Skin Micro-Organs of the Orinoco Lime
Treefrog (Sphaenorhynchus lacteus). Int J Mol Sci. 2018 Jul 25;19(8). pii: E2170.
doi: 10.3390/ijms19082170. PubMed PMID: 30044391; PubMed Central PMCID:

Pulido D, Rebollido-Rios R, Valle J, Andreu D, Boix E, Torrent M. Structural
similarities in the CPC clip motif explain peptide-binding promiscuity between
glycosaminoglycans and lipopolysaccharides. J R Soc Interface. 2017 Nov;14(136).
pii: 20170423. doi: 10.1098/rsif.2017.0423. PubMed PMID: 29187635; PubMed Central
PMCID: PMC5721150.

Arranz-Trullén J, Lu L, Pulido D, Bhakta S, Boix E. Host Antimicrobial
Peptides: The Promise of New Treatment Strategies against Tuberculosis. Front
Immunol. 2017 Nov 7;8:1499. doi: 10.3389/fimmu.2017.01499. eCollection 2017.
Review. PubMed PMID: 29163551; PubMed Central PMCID: PMC5681943.

Pulido D, Garcia-Mayoral MF, Moussaoui M, Velázquez D, Torrent M, Bruix M,
Boix E. Structural basis for endotoxin neutralization by the eosinophil cationic
protein. FEBS J. 2016 Nov;283(22):4176-4191. doi: 10.1111/febs.13915. Epub 2016
Oct 31. PubMed PMID: 27696685.

Pulido D, Prats-Ejarque G, Villalba C, Albacar M, González-López JJ, Torrent
M, Moussaoui M, Boix E. A Novel RNase 3/ECP Peptide for Pseudomonas aeruginosa
Biofilm Eradication That Combines Antimicrobial, Lipopolysaccharide Binding, and
Cell-Agglutinating Activities. Antimicrob Agents Chemother. 2016 Sep
23;60(10):6313-25. doi: 10.1128/AAC.00830-16. Print 2016 Oct. PubMed PMID:
27527084; PubMed Central PMCID: PMC5038260.

 Salazar VA, Arranz-Trullén J, Navarro S, Blanco JA, Sánchez D, Moussaoui M,
Boix E. Exploring the mechanisms of action of human secretory RNase 3 and RNase 7
against Candida albicans. Microbiologyopen. 2016 Oct;5(5):830-845. doi:
10.1002/mbo3.373. Epub 2016 Jun 8. PubMed PMID: 27277554; PubMed Central PMCID:

Pulido D, Arranz-Trullén J, Prats-Ejarque G, Velázquez D, Torrent M, Moussaoui
M, Boix E. Insights into the Antimicrobial Mechanism of Action of Human RNase6:
Structural Determinants for Bacterial Cell Agglutination and Membrane Permeation.
Int J Mol Sci. 2016 Apr 13;17(4):552. doi: 10.3390/ijms17040552. PubMed PMID:
27089320; PubMed Central PMCID: PMC4849008.

Prats-Ejarque G, Arranz-Trullén J, Blanco JA, Pulido D, Nogués MV, Moussaoui
M, Boix E. The first crystal structure of human RNase 6 reveals a novel
substrate-binding and cleavage site arrangement. Biochem J. 2016 Jun
1;473(11):1523-36. doi: 10.1042/BCJ20160245. Epub 2016 Mar 24. PubMed PMID:
27013146; PubMed Central PMCID: PMC4888456.

Salazar VA, Rubin J, Moussaoui M, Pulido D, Nogués MV, Venge P, Boix E.
Protein post-translational modification in host defense: the antimicrobial
mechanism of action of human eosinophil cationic protein native forms. FEBS J.
2014 Dec;281(24):5432-46. doi: 10.1111/febs.13082. Epub 2014 Oct 20. PubMed PMID:

Pulido D, Moussaoui M, Nogués MV, Torrent M, Boix E. Towards the rational
design of antimicrobial proteins: single point mutations can switch on
bactericidal and agglutinating activities on the RNase A superfamily lineage.
FEBS J. 2013 Nov;280(22):5841-52. doi: 10.1111/febs.12506. Epub 2013 Oct 8.
PubMed PMID: 23992292.

Torrent M, Pulido D, Valle J, Nogués MV, Andreu D, Boix E. Ribonucleases as a
host-defence family: evidence of evolutionarily conserved antimicrobial activity
at the N-terminus. Biochem J. 2013 Nov 15;456(1):99-108. doi: 10.1042/BJ20130123.
PubMed PMID: 23962023.

Pulido D, Torrent M, Andreu D, Nogués MV, Boix E. Two human host defense
ribonucleases against mycobacteria, the eosinophil cationic protein (RNase 3) and
RNase 7. Antimicrob Agents Chemother. 2013 Aug;57(8):3797-805. doi:
10.1128/AAC.00428-13. Epub 2013 May 28. PubMed PMID: 23716047; PubMed Central
PMCID: PMC3719706.

Boix E, Blanco JA, Nogués MV, Moussaoui M. Nucleotide binding architecture
for secreted cytotoxic endoribonucleases. Biochimie. 2013 Jun;95(6):1087-97. doi:
10.1016/j.biochi.2012.12.015. Epub 2012 Dec 26. Review. PubMed PMID: 23274129.

Torrent M, Pulido D, Nogués MV, Boix E. Exploring new biological functions of
amyloids: bacteria cell agglutination mediated by host protein aggregation. PLoS
Pathog. 2012;8(11):e1003005. doi: 10.1371/journal.ppat.1003005. Epub 2012 Nov 1.
PubMed PMID: 23133388; PubMed Central PMCID: PMC3486885.

García-Mayoral MF, Canales A, Díaz D, López-Prados J, Moussaoui M, de Paz JL,
Angulo J, Nieto PM, Jiménez-Barbero J, Boix E, Bruix M. Insights into the
glycosaminoglycan-mediated cytotoxic mechanism of eosinophil cationic protein
revealed by NMR. ACS Chem Biol. 2013 Jan 18;8(1):144-51. doi: 10.1021/cb300386v.
Epub 2012 Oct 9. PubMed PMID: 23025322.

Boix E, Salazar VA, Torrent M, Pulido D, Nogués MV, Moussaoui M. Structural
determinants of the eosinophil cationic protein antimicrobial activity. Biol
Chem. 2012 Aug;393(8):801-15. doi: 10.1515/hsz-2012-0160. Review. PubMed PMID:

Torrent M, Nogués MV, Andreu D, Boix E. The "CPC clip motif": a conserved
structural signature for heparin-binding proteins. PLoS One. 2012;7(8):e42692.
doi: 10.1371/journal.pone.0042692. Epub 2012 Aug 6. PubMed PMID: 22880084; PubMed
Central PMCID: PMC3412806.

Torrent M, Nogués MV, Boix E. Discovering new in silico tools for
antimicrobial peptide prediction. Curr Drug Targets. 2012 Aug;13(9):1148-57.
PubMed PMID: 22664076.

Boix E. Editorial: novel strategies for the design of therapeutic
antimicrobial peptides. Curr Drug Targets. 2012 Aug;13(9):1119-20. PubMed PMID:

Boix E, Pulido D, Moussaoui M, Nogués MV, Russi S. The sulfate-binding site
structure of the human eosinophil cationic protein as revealed by a new crystal
form. J Struct Biol. 2012 Jul;179(1):1-9. doi: 10.1016/j.jsb.2012.04.023. Epub
2012 May 9. PubMed PMID: 22579681.

Pulido D, Nogués MV, Boix E, Torrent M. Lipopolysaccharide neutralization by
antimicrobial peptides: a gambit in the innate host defense strategy. J Innate
Immun. 2012;4(4):327-36. doi: 10.1159/000336713. Epub 2012 Mar 21. Review. PubMed
PMID: 22441679.

Pulido D, Moussaoui M, Andreu D, Nogués MV, Torrent M, Boix E. Antimicrobial
action and cell agglutination by the eosinophil cationic protein are modulated by
the cell wall lipopolysaccharide structure. Antimicrob Agents Chemother. 2012
May;56(5):2378-85. doi: 10.1128/AAC.06107-11. Epub 2012 Feb 13. PubMed PMID:
22330910; PubMed Central PMCID: PMC3346588.

Torrent M, Di Tommaso P, Pulido D, Nogués MV, Notredame C, Boix E, Andreu D.
AMPA: an automated web server for prediction of protein antimicrobial regions.
Bioinformatics. 2012 Jan 1;28(1):130-1. doi: 10.1093/bioinformatics/btr604. Epub
2011 Nov 3. PubMed PMID: 22053077.


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