|Títol||Meiotic homologous chromosome alignment and its surveillance are controlled by mouse HORMAD1|
|Tipus de publicació||Journal Article|
|Any de publicació||2011|
|Autors||Daniel, K, Lange, J, Hached, K, Fu, J, Anastassiadis, K, Roig, I, Cooke, HJ, Stewart, FA, Wassmann, K, Jasin, M, Keeney, S, Tóth, A|
|Journal||Nature cell biology|
|Pàgines||599 - 610|
Meiotic crossover (CO) formation between homologous chromosomes (homologues) entails DNA double strand break (DSB) formation, homology search using DSB ends, and synaptonemal complex (SC) formation coupled with DSB repair. Meiotic progression must be prevented until DSB repair and homologue alignment are completed to avoid forming aneuploid gametes. Here we show that mouse HORMAD1 ensures that sufficient numbers of processed DSBs are available for successful homology search. HORMAD1 is needed for normal SC formation and for the efficient recruitment of ATR checkpoint kinase activity to unsynapsed chromatin. The latter phenomenon was proposed to be important in meiotic prophase checkpoints in both sexes. Consistent with this hypothesis, HORMAD1 is essential for the elimination of SC-defective oocytes. SC formation results in HORMAD1 depletion from chromosome axes. Thus, we propose that SC and HORMAD1 are key components of a negative feedback loop that coordinates meiotic progression with homologue alignment: HORMAD1 promotes homologue alignment and SC formation, and SCs down-regulate HORMAD1 function, thereby permitting progression past meiotic prophase checkpoints.